Table IV. Stomatal pore dimensions, guard cell pair dimensions, and guard cell dimensions in wild-type and xxt1 xxt2 plants during FC or ABA treatment.
Stomatal aperture, stomatal pore length, ratio of aperture to pore length, guard cell pair height, guard cell pair width, aspect ratio of guard cell pairs (height to width), ratio of aperture to guard cell pair width, guard cell diameter, and guard cell length were measured on a single stomate basis using epidermal peels from 3- to 4-week-old plants. Values are presented as means ± se (n > 80 stomata for each genotype at each time point from three experiments). Lowercase letters represent significantly different groups (P < 0.05, one-way ANOVA and Tukey’s test). ANOVA was performed within each treatment. NA, Not applicable.
| Treatment | Time after Treatment | Genotype | Average Stomatal Aperture | Average Stomatal Pore Length (μm) | Average Ratio of Aperture to Pore Length | Average Guard Cell Pair Height | Average Guard Cell Pair Width (μm) | Average Aspect Ratio of Guard Cell Pairs | Average Ratio of Aperture to Guard Cell Pair Width | Average Guard Cell Diameter | Average Guard Cell Length (μm) | Change in Average Guard Cell Length (μm) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| h | µm | µm | µm | |||||||||
| FC | 0 | Col-0 | 0.7 ± 0.0 a | 10.9 ± 0.2 a | 0.06 ± 0.00 a | 26.5 ± 0.2 a | 18.1 ± 0.1 a | 1.47 ± 0.01 a | 0.03 ± 0.00 a | 7.6 ± 0.1 a | 25.4 ± 0.2 a | NA |
| xxt1 xxt2 | 0.5 ± 0.0 a | 9.7 ± 0.2 b | 0.05 ± 0.00 a | 25.2 ± 0.2 b | 18.0 ± 0.1 a | 1.40 ± 0.01 c | 0.03 ± 0.00 a | 7.5 ± 0.1 a | 24.7 ± 0.2 a | NA | ||
| 2.5 | Col-0 | 2.4 ± 0.1 b | 12.2 ± 0.3 c | 0.23 ± 0.01 b | 26.8 ± 0.3 a | 20.4 ± 0.2 b | 1.32 ± 0.01 b | 0.12 ± 0.00 b | 7.5 ± 0.1 a | 26.7 ± 0.3 b | 1.3 | |
| xxt1 xxt2 | 2.2 ± 0.1 b | 9.9 ± 0.2 b | 0.25 ± 0.01 c | 24.1 ± 0.2 c | 19.9 ± 0.1 b | 1.21 ± 0.01 d | 0.12 ± 0.00 b | 7.6 ± 0.1 a | 25.0 ± 0.2 a | 0.3 | ||
| ABA | 0 | Col-0 | 3.1 ± 0.1 a | 10.8 ± 0.2 a | 0.25 ± 0.01 a | 24.6 ± 0.3 a | 20.0 ± 0.2 a | 1.24 ± 0.01 a | 0.14 ± 0.00 a | 7.5 ± 0.1 a | 25.1 ± 0.2 a | NA |
| xxt1 xxt2 | 2.5 ± 0.1 c | 10.1 ± 0.2 b | 0.20 ± 0.01 b | 24.0 ± 0.3 a | 19.1 ± 0.2 c | 1.25 ± 0.01 a | 0.12 ± 0.00 c | 7.4 ± 0.1 a | 25.1 ± 0.3 a | NA | ||
| 2.5 | Col-0 | 0.8 ± 0.1 b | 10.2 ± 0.2 a,b | 0.06 ± 0.00 c | 24.9 ± 0.3 a | 17.5 ± 0.2 b | 1.43 ± 0.02 b | 0.03 ± 0.00 b | 7.5 ± 0.1 a | 24.4 ± 0.2 a | −0.7 | |
| xxt1 xxt2 | 0.6 ± 0.0 d | 10.3 ± 0.2 a,b | 0.05 ± 0.00 c | 24.6 ± 0.3 a | 17.1 ± 0.2 b | 1.45 ± 0.02 b | 0.02 ± 0.00 d | 7.4 ± 0.1 a | 24.8 ± 0.2 a | −0.3 |