Table I. Purification of plant tubulins by the TOG column.
| Material | Culture Duration (Volume of Culture Medium) | Column Sizea | Sample Weight | Total Protein | Purified Tubulin | Percentage in Total Protein |
|---|---|---|---|---|---|---|
| g fresh wt | mg | μg | ||||
| Arabidopsis seedlings | S | 15 | 75 | 18 | 0.024 | |
| Arabidopsis MM2d cultured cells | 4 d (300 mL) | S | 26 | 211 | 590 | 0.28 |
| S | Flow throughb | 253 | 0.12 | |||
| Arabidopsis MM2d cultured cells | 4 d (200 mL) | M | 18 | 150 | 687 | 0.46 |
| Arabidopsis T87 cultured cells | 4 d (200 mL) | S | 19 | 96 | 224 | 0.23 |
| Arabidopsis Alex cultured cells | 3 d (100 mL) | S | 18 | 91 | 26 | 0.029 |
| Tobacco BY-2 cultured cells | 3 to 4 d (300 mL) | S | 10 | 89 | 244 | 0.27 |
| Tobacco BY-2 cultured cells | 5 d (100 mL) | S | 12 | 70 | 58 | 0.083 |
a
The resin volumes for the small (S) and medium (M) columns are 1 and 2 mL, respectively. bThe flow-through fraction of the above TOG column purification step was recovered and applied to a new TOG column.