Expression levels of miR163 and PXMT1 at different temperatures. A, Expression levels of pri-miR163 and PXMT1 at 22°C, 4°C, and 28°C. Etiolated seedlings (d) were incubated at different temperatures (22°C, 4°C, and 28°C) under the same light intensity (100 µmol m−2 s−1) as indicated (1, 6, and 24 h). Values were normalized to that of the d sample of each panel. Error bars indicate sd (n = 3). B, Small RNA gel-blot analysis showing miR163 expression at 22°C, 4°C, and 28°C. Total RNA was extracted from the samples described in A. U6 snRNA levels were used as a loading control.