Figure 4.

Regulated expression of PXMT1 by miR163 during seedling de-etiolation. A to F, Expression levels of pri-miR163, miR163, and PXMT1 in the wild type (WT; A), mir163 mutant miR163^KO (B), miR163 complementation line miR163^COM (C), miR163 overexpression line miR163^OE (D), PXMT1 RNAi line PXMT1^RNAi (E), and PXMT1 overexpression line PXMT1^OE (F). Four-day-old etiolated seedlings (d) were treated with W light as indicated (1, 6, and 24 h). All conditions for qRT-PCR and small RNA gel-blot analysis were identical to those described in Figure 1, A and C. Values in A to F were normalized to that of the d sample in the wild type (A). Error bars indicate sd (n = 3).