MiR163 is involved seedling root growth. A, Root phenotypes of the wild type (WT), mir163 mutant miR163KO, miR163 overexpression line miR163OE, PXMT1 overexpression line PXMT1OE, and PXMT1
RNAi line PXMT1RNAi. The seedlings were grown on Murashige and Skoog medium for 14 d under long-day conditions (16 h light/8 h dark). Scale bar = 1 cm. B, Primary root length of seedlings shown in A. C, Number of lateral roots of seedlings shown in A. D, Elongation/differentiation zone length of the wild type, mir163 mutant miR163KO, miR163 overexpression line miR163OE, PXMT1 overexpression line PXMT1OE, and PXMT1
RNAi line PXMT1RNAi. Blue line indicates the elongation/differentiation zone in root meristem. Scale bar = 0.2 mm. PM, Proximal meristem; EDZ, elongation/differentiation zone. E, Elongation/differentiation zone length shown in D. F, Number of cells in elongation/differentiation zone shown in D. B, C, E, and F, Error bars indicate the mean ± sd (n = 50) with biological replicates, and values (*) are statistically significant from the wild type based on Student’s t test (P < 0.001).