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. 2016 Jan 14;170(3):1772–1782. doi: 10.1104/pp.15.01188

Figure 6.

Figure 6.

MiR163 plays a role in seed germination. A, Seed germination of the wild type, mir163 mutant miR163KO, miR163 complementation line miR163COM, miR163 overexpression line miR163OE, PXMT1 RNAi line PXMT1RNAi, and PXMT1 overexpression line PXMT1OE. Seed germination was scored by radicle emergence at different time points (36, 42, 48, and 54 h) under continuous light (100 µmol m−2 s−1). Seed numbers (n) are represented below the x axis. Error bars indicate sd (n = 3). B, Histochemical localization of GUS activity in germinating transgenic Arabidopsis seeds carrying pri-miR163 promoter::GUS-GFP transgene. GUS staining was performed with de-etiolated seedlings indicated (1, 6, and 24 h). C, Expression levels of pri-miR163 and PXMT1 during seed germination or seedling de-etiolation. Arrows indicate sampling points. Values were normalized to that of the d0 sample of each group with Actin8 expression level. Error bars indicate sd (n = 3).