Figure 1.

Expression patterns of TaTOC-A1, TaTOC-B1, and TaTOC-D1 in different wheat organs. A, Total RNAs were isolated from various organs of hexaploid wheat at ZT12 under 12-h-light/12-h-dark conditions. Expression of TaTOC-A1, TaTOC-B1, and TaTOC-D1 expression was analyzed by RT-PCR. B, Real-time qRT-PCR was performed using homolog-specific primers of TaTOC-A1, TaTOC-B1, and TaTOC-D1 under highly stringent conditions. The TaActin gene was used as an endogenous control. The data are the means from three replicates. C–I, In situ localization of the TaTOC1 mRNAs in the vegetative shoot apices, inflorescence primordia, and leaves of wheat. C-E, Longitudinal sections of the vegetative shoot apex and an inflorescence primordium. F and G, Transverse sections of the leaf primordia and young leaves in a seedling at day 1 after germination. H, Transverse section of young leaf from seedlings grown for 14 d. I, An enlarged view of the leaf middle vein in H. D, E, H, and I, Leaf sections that were hybridized with a TaTOC1 antisense mRNA probe. C and F, Controls hybridized with a sense probe. AM, apical meristem; Co, coleoptile; In, internodes; L, leaf; LB, lateral bud; LE, lower epidermal; LP, leaf primordia; LS, leaf sheaths; MR, mature roots; Me, mesophyl; ML, mature leaves; N, nodes; P, phloem; SA, shoot apices; SAM, shoot apical meristem; Spi, spikelets; UE, upper epidermal; St, stoma; X, xylem; YL, young leaf; YR, young root. Bars in C–E, 200 μm; in F and G, 350 μm; in H and I, 15 μm.