Figure 2.

GRXS15^KD T-DNA insertion knockdown and phenotype. A, Relative expression level determined by qPCR of GrxS15 transcript in wild type, GRXS15^KD, GRXS15^amiR, and GRXS15^comp. Shown is average ± SD, n = 3. B, MRM analysis using two specific peptides for GrxS15 of whole tissue mitochondrial protein in wild type, GRXS15^KD, GRXS15^amiR, and GRXS15^comp. Peak area was normalized to the total MS2scan and standardized to wild type. Shown is median ± sem, n = 3. Student’s t test has been performed, ** indicates P < 0.001, *** indicates P < 0.0001. C, Western blot analysis with primary antibody raised against GrxS15 using isolated mitochondria from whole tissue of wild type, GRXS15^KD, GRXS15^amiR, and GRXS15^comp. Shown is an exemplary image with values obtained from densitometry analysis of all replicates. Median and SEM are displayed, n = 4. Student’s t test has been performed, * indicates P < 0.1, ** indicates P < 0.0001.