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. 2016 Jan 27;170(3):1675–1683. doi: 10.1104/pp.15.01501

Figure 2.

Figure 2.

SEU physically associates with promoter regions of SHR, SCR, and SCL3 and positively regulates their expression. A to H, Expression of SHR::H2B-YFP (A and B), SCR::H2B-YFP (C and D), SCL3::GUS (E and F), and SCL3::SCL3-GFP (G and H) in root tips of Col-0 (A, C, E, and G) and seu-3 (B, D, F, and H) seedlings. Scale bars = 50 μm. I, Yeast one-hybrid assay. The boundaries of promoter regions of SCR, SHR, and SCL3 used for reporter constructs are shown. Yeast cells cotransformed with each combination of reporter and SEU-GAL4-AD constructs (+) were tested for β-galactosidase assay (blue color). Yeast cells transformed only with each of the reporter constructs were used as controls (−). J to L, ChIP-qPCR assay. The relative ChIP recovery of each region in the SEU::SEU-GFP line was expressed as percentage of input DNA (y axis). Error bars represent sd of three independent experiments. Bars with different letters are significantly different at P < 0.05, ANOVA. nA, No antibody; Ab, GFP antibody.