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. 2016 Jan 6;170(3):1732–1744. doi: 10.1104/pp.15.01859

Figure 6.

Figure 6.

Transcriptional regulation of ERF.E1, ERF.E2, and ERF.E4. A, Presence of putative RIN-binding sites [CArG, C(C/T)(A/T)6(A/G)G; NAC-binding site (NBS), CATGTG; and ERE, A(A/T)TTCAAA] and a putative ERF-binding element (DRE) in the promoters of ERF genes. The cis-acting elements identified are represented by black bars and localized from ATG. B, Transactivation of ERF promoters by RIN. Protoplasts were cotransfected with the GFP reporter fused to the promoters of ERFs (ERF.E1, E2, E4, and F2) and an effector plasmid expressing RIN under the control of the 35S promoter. In the transactivation assay of the pGCC synthetic promoter (4× GCC box) by RIN and ERF.B3, protoplasts were cotransfected with the GFP reporter fused to the synthetic promoter and an effector plasmid expressing either RIN or ERF.B3 under the control of the 35S promoter. Gray bars correspond to the control for each GFP reporter. Values represent means of three biological replicates. Error bars represent sd. C, Transactivation of ERF.E1 promoters by ERF.E2 and ERF.E4. Protoplasts were cotransfected with the GFP reporter fused to the promoter of ERF.E1 and an effector plasmid expressing either ERF.E2 or ERF.E4 under the control of the 35S promoter. Values represent means of three biological replicates. Error bars represent sd. *, P < 0.01; **, P < 0.001; and ***, P < 0.0001.