Table I. Amount of CA released by mild alkaline hydrolysis of the mature stems of Arabidopsis wild-type and BdPMT1- and BdPMT2-expressing lines under the control of the AtC4H promoter and of the corresponding purified DL fractions.
Data from grass samples are given for comparison purposes. The data for Arabidopsis CW represent means (and sd) from three different plant pools (about 20 plants per pool) for each line. Asterisks indicate significant differences (one-way ANOVA) compared with the value of the corresponding control at P < 0.001. For grass CWs, data are means (and sd) from analytical triplicates. For Arabidopsis samples, DL isolation was performed from the pool of biological triplicates, and each DL fraction was then subjected to analytical replicates (n = 2 or 3). For grass DL samples, each DL fraction was analyzed as analytical triplicates.
| Plant and Genotype |
CA from Whole CWs |
CA from Corresponding DL Fractions |
|---|---|---|
| mg g−1 | ||
| Arabidopsis extract-free mature stems | ||
| Wild-type Col-0 | 0.016 (0.002) | 0.93 (0.10) |
| BdPMT1/wt line1-3 | 7.84 (0.20)* | 48.0 (4.1) |
| BdPMT1/wt line12-8 | 12.57 (0.39)* | 66.3 (1.4) |
| Wild-type Col-0 | 0.010 (0.004) | 1.34 (0.04) |
| BdPMT2/wt line2-3 | 13.36 (0.81)* | 71.9 (4.3) |
| BdPMT2/wt line7-7 | 12.74 (0.23)* | 76.6 (2.1) |
| Grass extract-free mature stems | ||
| Maize stem (F2 line, silage stage) | 17.39 (0.66) | 119.8 (2.1) |
| B. distachyon Bd21-3 mature stems | 8.92 (0.63) | 35.4 (1.0) |
| Wheat straw (cv Champlein) | 4.76 (0.10) | 23.8 (1.4) |