TF analyses among the brace root specific nitrate-responsive genes. A, Distribution of the 132 nitrate-responsive TFs according to families. TFs were classified according to PlantTFDB3.0 database annotation. B, Prediction of connectivity of 132 TFs identified from brace root specific nitrate-responsive genes using STRING v10. Color codes indicate expression values of transcripts normalized as log2 algorithm of fold changes. A green line indicates text mining evidence, a purple line indicates experimental evidence, a blue line indicates database evidence, and a black line indicates coexpression evidence. Clustered groups associated with “MADS box,” “MYB,” and “CPP” families were highlighted accordingly. Outlines of nodes representing “classical” maize transcription factors were thickened.