Figure 5.

Uptake and incorporation of ADP-[¹⁴C]glc into starch by purified amyloplasts from wild-type (WT) and transgenic rice seeds. ADPglc import into amyloplasts is shown for the wild-type, GlgC (CS8), ZmBT1 (AT5), and GlgC/ZmBT1 (CS8/AT5) lines in the presence or absence of adenylates. Amyloplasts were preloaded with buffer (black vertical lines), 20 mm ATP (blue horizontal lines), 20 mm ADP (red checkered lines), or 20 mm AMP (green crosswise lines) for 10 min. Uptake experiments were performed at 30°C for 30 min. Data represent means ± se of three independent experiments. FW, Fresh weight.