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Pulse-chase studies using ADP-[14C]glc to assess starch turnover in intact amyloplast. Wild-type (WT; A) and GlgC (CS8; B) amyloplasts, preloaded with ADP, were incubated in the presence of 0.1 mm ADP-[14C]glc. After 20 min of incubation, a 100-fold excess (10 mm) of unlabeled ADPglc was added to the amyloplast mixture, and the extent of radioactivity in starch was assessed. Data represent means ± se of three independent experiments. FW, Fresh weight.
