Table III. Uptake and incorporation of ADP-[14C]glc into starch by purified intact amyloplasts in the presence or absence of the counter-exchange ADP.
Total starch synthase activity was estimated by lysing the amyloplasts and measuring the amount of incorporation of ADP-[14C]glc into starch. Each value represents the mean ± se of three independent experiments.
| Lines | Intact |
Starch Synthase Activity |
||
|---|---|---|---|---|
| Buffer | +ADP | Buffer | +ADP | |
| nmol h−1 g−1 fresh wt | ||||
| Wild-type (cv Kitaake) | 235 ± 33 | 437 ± 45 | 145 ± 13 | 140 ± 11 |
| ZmBT1 (AT5-1) | 445 ± 43 | 850 ± 47 | 153 ± 11 | 152 ± 10 |
| ZmBT1 (AT5-19) | 450 ± 34 | 860 ± 39 | 150 ± 9 | 154 ± 11 |
| GlgC (CS8) | 265 ± 23 | 440 ± 63 | 125 ± 7 | 120 ± 8 |
| GlgC/ZmBT1 (CS8/AT5-18) | 466 ± 41 | 881 ± 70 | 147 ± 12 | 141 ± 16 |
| GlgC/ZmBT1 (CS8/AT5-27) | 440 ± 44 | 897 ± 71 | 144 ± 11 | 154 ± 19 |
| GlgC/ZmBT1 (CS8/AT5-37) | 454 ± 50 | 985 ± 72 | 149 ± 13 | 139 ± 17 |
| Wild-type (cv TC65) | 245 ± 33 | 480 ± 49 | 125 ± 18 | 135 ± 15 |
| EM1093 (cv TC65) | 49 ± 7 | 51 ± 6 | 43 ± 5 | 41 ± 3 |