| Protocol 8 Western Blot Antibody Multiplexing | ||||
|---|---|---|---|---|
| Protein of interest | Loading Control | |||
| Combination | Description | Working Conc. | Description | Working Conc. |
| 1 | Rabbit phospho-IGF-1R (Y1165/1166) (95 kDa) |
1:500-1:2000 | Mouse anti-ERK1/2 (42/44 kDa) |
1:2000 |
| 2 | Rabbit phospho-IGF-1R (Y1165/1166) (95 kDa) |
1:500-1:2000 | Mouse anti-IGF-1R (95 kDa) |
0.1–2 µg/ml |
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