Figure 2. The localization of endosomal proteins in pan1-18TA cells.
(A) Localization of GFP-tagged endosomal proteins in pan1-18TA. pan1-18TA cells expressing Abp1-mCherry and GFP-tagged endosomal proteins were grown to early to mid-logarithmic phase in YPD medium at 25°C and observed by fluorescence microscopy. Merged images of GFP and mCherry channels are shown in the lower panel. Arrowheads indicate examples of colocalization. Scale bar, 2.5 μm. (B) Quantification of actin clumps including GFP-tagged endosomal proteins. The percentages were calculated as the ratio of actin clumps (n = 100) colocalizing with each protein in each experiment. Error bars indicate the standard deviation (SD) from at least three independent experiments.
Figure 2—figure supplement 1. Localization of endosomal proteins in wild-type cells.
Cells expressing each GFP-tagged endosomal protein were grown to early to mid-logarithmic phase in YPD medium at 25°C and observed by fluorescence microscopy.
Scale bars, 2.5 μm.