Figure 7. Model of the actin cable-mediated endocytic pathway.
Unphosphorylated Pan1p on an endocytic vesicle binds to actin to fix the vesicle to the actin cable. After being pinched off from the membrane, the endocytic vesicle moves into the cytosol as a result of actin cable flow, and then, interacts with the early endosome via potential tethering protein. Pan1p phosphorylation by Ark1/Prk1 kinases causes dissociation of coat proteins and the actin cable from the endocytic vesicle, making it possible for the vesicle to fuse to the endosome. This also results in the dissociation of the actin cable and the early endosome, which then moves to the vacuolar membrane, and matures into a late endosome.