Author response image 1. Injury decreases the CSA in regenerating myofibers to the same extend in WT and TBP2KO.
(A) Muscle histology: Hematoxilin & Eosin staining of WT muscle crosssections at different time point of regeneration. (B) Immunohistochemistry: cross-sections of WT and TBP2_KO muscles were stained with anti-collagen1 and anti-collagen-3 antibodies (red) to visualize clearly the myofibers. The nuclei were counterstained with DAPI. (C) Mean of all cross-section areas (CSA) quantified by ImageJ for each muscle was compared between injured and unperturbed muscle, the ratio is plotted. D) The centrally nucleated myofibers in 1B were quantified.