Figure 6. HMM modeling of refolding FRET trajectories.
(A) Four-state HMM fit to a 30-second portion of concatenated refolding FRET trajectories in the presence of 5 µM TPP. (B) Top: Reaction diagram for the four-state, sequential HMM model, with states UF and A (donor-fluorescing), and B and C (acceptor-fluorescing). Bottom: table summarizing FRET values obtained 5 µM and 50 µM TPP concentrations for each state. (C) Table summarizing directional rates for transitions between HMM states A, B, and C. The yellow icon indicates the TPP-dependent transition rate.