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. Author manuscript; available in PMC: 2016 Dec 2.
Published in final edited form as: Neuron. 2015 Nov 19;88(5):879–891. doi: 10.1016/j.neuron.2015.10.026

Figure 6. In vivo Photo-control of Visual Responses in the Mouse Cortex.

Figure 6

(A) Schematic illustration of the experimental procedures.

(B) Two-photon image of a recorded PV+ neuron. The cell was identified by the co-expression of tdTomato (red, marker of PV+ cell) and eGFP (green, marker of LiGABAR expression).

(C) Experimental sequence. The raster plots and peristimulus time histograms show the spike activity of a PV+ neuron before any conditioning illumination (black), and after a 10-sec exposure to either 390 nm (violet) or 470 nm (green) light.

(D) Summary of visually evoked spike activities in PV+ neurons (circles), showing higher firing rates when LiGABAR was antagonized (dark and 470 nm) than when it was relieved from antagonism (390 nm). n = 7 cells from 4 mice. Control experiments with PTL treatment alone (squares; n = 6 cells from 2 mice) or viral injection alone (triangles; n = 9 cells from 2 mice) show no significant difference in spike activities after exposure to 390 nm vs. to 470 nm light. Data are plotted as mean ± SEM.