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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biochemistry. 2016 Feb 17;55(8):1214–1225. doi: 10.1021/acs.biochem.5b01329

Table 2.

SPR Analysis of W55D and Variantsa

protein kon (M−1 s−1) koff (s−1) Koff/kon = Kd(app) (nM) χ2 Kd(app) (nM)
W55D 5.2 × 104 1.6 × 10−3 30.0 5.4 580 ± 94
W55D
 R9A		 3.8 × 104 6.4 × 10−4 17.0 17.0 660 ± 200
W55D
 K25A		 5.0 × 104 1.9 × 10−3 38.0 7.6 1090 ± 140
W55D
 R23A/R43A		 1.8 × 104 2.2 × 10−3 120 0.4 1800 ± 460
CC3 2.7 × 104 1.7 × 10−3 63.0 0.7 1100 ± 480
XCL1
 WT		 2.6 × 104 1.2 × 10−3 46.1 1.9 420 ± 40
a

Kinetic on and off rates derived from simultaneous curve fitting of a 1:1 binding model at protein concentrations of 100, 250, 500, 750, and 1000 nM. Kd values were calculated through both kinetic (fourth column) and steady state (sixth column) analysis.