Figure 5.

EGb 761 pretreatment enhances neurogenesis. Mice pretreated with EGb 761 after OVX for 7 days prior to pMCAO and allowed to survive additional 7 days without drug treatment were sacrificed at day 8. BrdU was injected 4 h after pMCAO and then daily for 7 days (schematic diagram of Fig. 1A). A) Triple immunofluorescence staining of DAPI (blue), BrdU (green), and netrin-1 (red) and overlay of brain sections (6 µm) of female mice brains. B) The image represents the area from which the netrin+BrdU cells were counted; the image was taken from Cytation 5 immunofluorescence microscope (Biotek, Winooski, VT); the ischemic region is in the oval shaped (dotted) area, whereas as the NSPCs are counted from the rectangular area. C) The number of netrin+BrdU positive cells were counted near the peri infarct area and the graph was plotted. Data are expressed as mean±SEM; p < 0.05 vs. sham; ***p <0.05 vs. Intact, p< 0.05 vs. Veh/OVX; **p <0.05 vs. EGb 761/OVX (n=3 each group). The pictures were taken by Nikon Eclipse Ti microscope using × 100 objectives.