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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Eukaryot Microbiol. 2015 Oct 19;63(2):233–246. doi: 10.1111/jeu.12272

Figure 5.

Figure 5

Immunofluorescence labeling showing that CpACS1 protein is an apical protein and mainly localized in the anterior region of C. parvum sporozoites, merozoites and intracellular parasites. Images were taken by differential interference contrast microscopy (DIC), fluorescence microscopy using filter sets for 4′,6-diamidino-2-phenylindole (DAPI) staining nuclei and tetramethylrhodamine (TRITC) labeling CpACS proteins, and superimposed (Merged). Abbreviations: a, anterior region of sporozoites or merozoites; p, posterior region of sporozoites or merozoites; em, early stage meronts; m, merozoites; r, meronts; v, parasitophorous vacuole membrane (PVM). Bars = 5 μm.