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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Clin Cancer Res. 2015 Oct 12;22(5):1211–1221. doi: 10.1158/1078-0432.CCR-15-0663

Figure 4. Mcl-1 plays an important role in ATRA-induced differentiation and apoptosis in HL-60 cells.

Figure 4

HL-60 cells were treated with 1μM ATRA for the indicated times and the percent of differentiated cells was determined by measuring CD11b expression (A) and of apoptotic cells using Annexin V (B). HL-60 cells were treated with 1μM ATRA for the indicated times and the levels of Mcl-1, Bcl-2, cleaved caspase-9,-8,-3, PARP and β-actin were determined using specific antibodies with Western blot (C). HL-60 cells transfected with Mcl-1 siRNA or control siRNA were treated with 1μM ATRA for 2 days. The protein levels of PARP, Mcl-1, Bcl-2 and β-actin were determined using specific antibodies with Western blot (D) and the percent of apoptotic cells was determined using FACS of cells stained with Annexin V-FITC (E).