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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Feb 18;14(8):1901–1915. doi: 10.1016/j.celrep.2016.01.067

Figure 2. Hox Genes Govern Limb Innervation Pattern and MN Pool Organization.

Figure 2

(A–B) Wholemount GFP staining of control Hb9::GFP mice at e12.5 and e13.5. Schematic representations of innervation pattern are shown in right panels and motor nerves are color coded. Arrows indicate region where nerve diameters were measured. (C–H) Forelimb innervation patterns in Hoxc6, Hoxc8 and HoxC mutants at e12.5 and e13.5. Arrows and arrowheads indicate major nerve defects. Scale-bars represent 500 μm. Innervation patterns in control littermates are shown in Figure S2. (I) Quantification of muscle nerve diameters in indicated Hox mutant lines. Data are shown as mean +/− S.E.M. (J–P) Retrograde labeling of MNs in Hoxc8 mutants. Hoxc8 mutants display defects in the organization of suprascapular, lateral pectoral, medial anterior thoracic, median and ulnar MNs. (Q–S) Retrograde labeling of MNs projecting along the lateral pectoral, medial anterior thoracic and radial nerves in Hoxc6 mutants. The position and organization of MNs projecting along the suprascapular and musculocutaneous nerves are not affected in Hoxc6 mutants (Figure S2K, L). See also Figure S2.