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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Feb 18;14(8):1901–1915. doi: 10.1016/j.celrep.2016.01.067

Figure 5. Hox and Meis Interactions Govern Ret Expression in LMC Neurons.

Figure 5

(A–D) Electroporation of Hoxc6-pCAGGs induces Ret and Pea3 expression at thoracic levels and Gfrα1 at rostral cervical levels. Hoxc6 and Isl1/2 costaining is shown to indicate electroporated MNs. Arrow in panel A indicates electroporated side of embryo. (E–H) Electroporation of Hoxc8-pCAGGs induces Ret, Gfrα4 and Scip expression at thoracic levels. (I–L) Misexpression of Meis1 represses Ret, Gfrα1 and Gfrα4 in caudal LMC neurons. (M–R) Expression of Meis1/2 and Lhx1 in chick and mouse lumbar LMC neurons. In both chick and mouse Meis1/2 expression is reduced in lateral Lhx1+ MNs, which express high levels of Ret. (S–V) Electroporation of Hoxc10 and Hoxd10 induces expression of Ret at thoracic levels. (W,X) Meis1 misexpression represses Ret in lumbar LMC neurons. (Y) Summary of Ret regulation by Hox/Hox co-factors in brachial LMC neurons. (Z) Summary showing regulation of Ret by Hox/Hox co-factors at lumbar levels. See also Figure S5.