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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Feb 18;14(8):1901–1915. doi: 10.1016/j.celrep.2016.01.067

Figure 7. Ret and Gfrα3 are Required for MN Pool Differentiation and Connectivity.

Figure 7

(A) Pea3 expression is reduced in brachial LMC neurons in Ret mutants at e12.5. (B) Quantification of the number and distribution of Pea3+ MNs in Ret−/− and control mice. (C) The number of Scip+ MNs increases in Ret−/− mice. (D) Quantification of the number and distribution of Scip+ MNs. (E,F) Wholemount GFP staining showing defects in CM muscle innervation in Ret−/− embryos (arrow) at e12.5. (G,H) Forelimb innervation at e13.5 showing failure of ulnar MNs to project distally in Ret−/− embryos (arrow). Arrows in control diagram indicate region where nerve diameters were measured. (I–K) Disorganization of MNs in Ret−/− mice shown by retrograde labeling of MNs projecting along the medial anterior thoracic, median and ulnar nerves. (L,M) Forelimb innervation pattern in Gfrα3+/+ and Gfrα3−/− mice at e13.5 showing thinning of the median and ulnar nerves. Scale-bars represent 500 μm. (N) Quantification of muscle nerve diameters expressed as a ratio between the radial and ulnar nerves in Ret and Gfrα3 mutants and controls at e13.5. See also Figure S7.