Intra-RMTg AMPA injection significantly decreases ethanol consumption and preference. Bilateral intra-RMTg AMPA (16.8 ng/300 nl/side, 0.3 mM) injection significantly decreased ethanol consumption (A), preference for ethanol (B) and total fluid intake (D), but not the water intake (C) measured 30 minutes after the onset of the ethanol drinking session, compared to Baseline or aCSF treatment group. Conversely, there was no significant change in ethanol intake (F), preference for ethanol (G), water intake (H) and total fluid intake (I) measured 24 h after the onset of ethanol drinking. The values are mean ethanol ± SEM (One-way RM ANOVA by Bonferroni post hoc test). * p < 0.05; *** p < 0.001 significant difference compared with Baseline or aCSF injection, n=8.