Intra-RMTg muscimol injection significantly increases ethanol consumption and preference. Muscimol (18.5 ng/125 nl/side, 1.3 mM) significantly increased ethanol consumption (A and E) and preference (B and F), but decreased water intake (C and G), without changing the total fluid intake (D and H) at either 30 min or 24 h after the onset of the ethanol drinking session. Muscimol did not alter ethanol intake 48 h after the onset of ethanol drinking. Values are mean ± S.E.M., (One-way repeat measure ANOVA followed by bonferroni post hoc test; n=8 for 30 min and 24h test). * p < 0.05 and ** p < 0.01 vs. Baseline, aCSF or 48 hours.