Intra-RMTg muscimol injection increases ethanol seeking. Muscimol (18.5 ng/125 nl/side, 1.3 mM) robustly elevated the number of active lever pressing (A). Data analysis within 30 min (bin=5 min) revealed that muscimol significantly increased active lever pressing during the initial 10 min (B). Muscimol injection increased the number of head entries into the ethanol port (C) and ethanol intake (D) during the 30-min session. Values are mean ±S.E.M. (one-way or two-way RM ANOVA followed by Bonferroni post hoc test; n=8). * p < 0.05 and *** p<0.001, compared with Baseline, aCSF or 24 h post-test; Δp <0.001, compared with aCSF; ### p<0.001, compared with baseline, aCSF or 24 h post-test on inactive lever pressing.