A, Atgl was effectively knocked down with ATGL-targeting lentiviral shRNA. Top, real-time PCR assay, bottom, Immunoblot. Bars are the average of biological triplicates and error bars are standard deviation. * P < 0.05. B, Left are representative plates from foci formation assays comparing the ability of HRAS expression plasmid to transform G0S2 null cells stably transduced with either shATGL or shControl lentivirus (top) or co-transfected with HRAS and either shATGL or shControl expression plasmids (bottom). Right, is the average of biological triplicate determinations and error bars are standard deviation. The experiments were repeated three times with similar results. C, G0S2 null MEFs were transfected with HRAS and the following day treated with either 40 µM of ATGL inhibitor atglistatin or DMSO (Vehicle) every 2 days. Left are representative plates. Right is the average of biological triplicate determinations and error bars are standard deviation. The experiment was repeated two times with similar results.