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One-pot three-enzyme (OP3E) synthesis of α1–2-linked fucosides.
| Acceptor | Product | Yield (%) | Amount (mg) |
|---|---|---|---|
| Galβ1–3GlcNAcβProN3 | Fucα1–2Galβ1–3GlcNAcβProN3 (1) | 96 | 50.5 |
| Galβ1–3GlcNAcαProN3 | Fucα1–2Galβ1–3GlcNAcαProN3 (2) | 95 | 51.3 |
| Galβ1–3GalNAcαProN3 | Fucα1–2Galβ1–3GalNAcαProN3 (3) | 95 | 35.7 |
| Galβ1–3GalNAcβProN3 | Fucα1–2Galβ1–3GalNAcβProN3 (4) | 98 | 43.8 |
| LNT | Fucα1–2LNT or LNFP I (5) | 94 | 68.1 |
| 95 | 1,146 |
