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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 17;1863(4):562–571. doi: 10.1016/j.bbamcr.2015.12.008

Fig. 6.

Fig. 6

Suppression of LGALS3 reduces BCL2 gene expression in OCI-AML3 but less so in THP-1 cells. RNA from OCI-AML3 transductant cells with either LKO vector control shRNA or LGALS3 shRNA (A) or LKO vector control shRNA or LGALS1 shRNA (B) was isolated, cDNA produced, and mRNA levels of BCL-2, BCL-XL, MCL-1, and ABL1 were determined by qRT-PCR and levels normalized to ABL-1 as described in “Materials and Methods”. C. Protein was isolated from OCI-AML3 and THP-1 transductant cells with either LKO vector control shRNA or LGALS3 shRNA. Immunoblot analysis was performed with antibodies against MCL-1, BCL2 and Tubulin. Ratio of protein expression to Tubulin loading control was determined by densitometry using LiCor imager.