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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Clin Cancer Res. 2015 Oct 16;22(5):1150–1160. doi: 10.1158/1078-0432.CCR-15-1522

Figure 1. MDM2 amplification and expression in DDLPS cell panel.

Figure 1

(A) Representative images of fluorescence in situ hybridization of the 12q13~15 amplicon in a cell line panel (MDM2: red signal and CEP12: green signal); DDLPS: Lipo246, Lipo224, Lipo863, Lipo815, Lipo224B, LPS141; Pleomorphic LPS: SW872, PLS1, LiSa2. (B) q-PCR was performed for MDM2 genomic amplification on a large DDLPS cell line panel, along with hMSC-AT as a normal control (C) q-PCR analyzed MDM2 mRNA expression levels in DDLPS cell lines, hMSC-AT, and in two pleomorphic LPS cell lines, PLS1 and LiSa2. (D) Western blot analysis of MDM2 protein expression in DDLPS cell lines versus control cell lines.