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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biomaterials. 2016 Feb 4;86:1–10. doi: 10.1016/j.biomaterials.2016.01.062

Figure 7. Effect of shape transition on cardiomyocyte vinculin orientation and shape.

Figure 7

(A) Representative image of NRVMs on SMP substrates before (A) and after (B) shape transition stained for vinculin (red) (scale bar = 10µm). (C, D, and E) Vinculin orientation distribution on permanent and SMP substrates. (F) Area of vinculin staining on permanent (mean ± standard error mean = 1.28±0.030) and SMP substrates before (1.49 ± 0.034) and after (mean = 1.434 ± 0.032) shape transition (n=1000 for all conditions). (G) Aspect ratio of vinculin staining on permanent (2.35 ±0.017) and SMP substrates before (2.46 ± 0.020) and after (2.307 ± .019) shape transition (no shape transition, n = 2028; before shape transition, n=1997; after shape transition, n=1447; * = p-value<0.001; * = p-value<0.0001).