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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Cancer Res. 2015 Dec 30;76(5):1055–1065. doi: 10.1158/0008-5472.CAN-14-3630

Figure 2. Immunoglobulin expression by In situ hybridization.

Figure 2

(A,B) Shown is the expression of kappa light chain mRNA in a representative prostate tumor from a current smoker in two different fields. In situ hybridization (ISH) depicts kappa light chain mRNA expression in lymphocytic infiltrates. The dark blue nitroblue tetrazolium chromogene reveals the predominant stromal localization of the immunoglobulin-positive B lymphocytes. Counterstain: Haematoxylin. (C,D) Analysis of ISH results. Numbers of Ig lambda- and Ig kappa-positive B lymphocytes correlated with smoking status [Spearman rank correlation test (Ptrend) for current (n = 6), past (n = 7), never (n = 9) smokers] and were increased in current smokers versus never/past smokers (Wilcoxon rank-sum test). ISH-positive lymphocytes were counted per 250x field. Shown are box plots with minimum and maximum values (whiskers) and the median as line in the box.