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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Neurochem Res. 2016 Jan 4;41(0):307–315. doi: 10.1007/s11064-015-1801-0

Figure 2.

Figure 2

Live/Dead Assay after in vitro trauma (5 atm) in cultured neurons. A) Control (non-traumatized sham) shows baseline live (calcein-AM-positive green fluorescence) and dead (ethidium homodimer-1-postive red fluorescence) cells in non-traumatized control neurons. B). Traumatized neurons at 18 after trauma showed a similar live/dead cell population as observed in sham control. C) Traumatized neurons at 24 h after trauma showed significant cell death, as compared to sham control. Green (calcein-AM) and Red (ethidium homodimer-1). Scale bar = 35 μm.