Table 3. Rate constants in different buffers (+100 mM NaCl, pH 8).
| buffer | pKa | RuH107YOH109 | RuH124YOH122 |
|---|---|---|---|
| acetate | 4.76 | (1.3 ± 0.3) × 104 | (5.0 ± 0.5) × 104 |
| imidazole | 7.05 | (1.7 ± 0.2) × 104 | (1.0 ± 0.2) × 105 |
| Tris | 8.07 | (1.7 ± 0.2) × 104 | (1.6 ± 0.2) × 105 |
| borate | 9.14 | (1.8 ± 0.2) × 104 | (1.7 ± 0.2) × 105 |