Gene counts for select genes and gene families in Anopheles gambiae (Order: Diptera), Aedes aegypti (Diptera), Drosophila melanogaster (Diptera), Pediculus humanus humanus (Siphonaptera), Manduca sexta (Lepidoptera), Tribolium castaneum (Coleoptera), Apis mellifera (Hymenoptera), and Acyrthosiphon pisum (Hemiptera). The gene number data was collected from (Cao et al., 2015; Chevignon et al., 2015; Evans et al., 2006; Gerardo et al., 2010; He et al., 2015; Kim et al., 2011; Sackton et al., 2007; Waterhouse et al., 2007; Zhang et al., 2015; Zou et al., 2007), with additional searches in https://www.vectorbase.org and http://metazoa.ensembl.org. The evolutionary relationships between the species, depicted at the top of the figure, are based on (Trautwein et al., 2012). Gene numbers can vary slightly depending on the analysis. Abbreviations: PRR, pattern recognition receptor; Toll (vertical orientation), Toll pathway; Imd (vertical orientation), Imd pathway; Effector, effector peptides and proteins; PGRP, peptidoglycan recognition protein; FREP, fibrinogen-related protein; βGRP, β-1,3 glucan recognition protein; GNBP, Gram(−) binding protein; CTL, C-type lectin; PPO, pro-phenoloxidase.