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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Cancer Res. 2015 Dec 16;76(5):1146–1157. doi: 10.1158/0008-5472.CAN-15-0442

Figure 4. Effect of ODC expression on anchorage-dependent and -independent colon cancer cell growth.

Figure 4

(A) ODC expression was analyzed by Western blot in HCT116 colon cancer cells expressing shMock, shODC + shMock, or shODC + ODC. (B) ODC activity was assessed as the release of L-[1-C14] ornithine. (C) Anchorage-dependent cell growth was measured by MTS assay. (D) Anchorage-independent colon cancer cell growth was analyzed. Colonies were counted using a microscope and the Image-Pro PLUS (v6) computer software program. Data shown in B, C and D are represented as means ± S.D. of triplicate values from 3 independent experiments and the asterisk (*) indicates a significant (p < 0.05) difference versus shODC + shMock cells.