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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Feb 18;14(8):1841–1849. doi: 10.1016/j.celrep.2016.01.069

Figure 4. Kur Interacts with PCP Components.

Figure 4

(A–C) Low concentration of inv or kur MO produce few defects but, in combination, produce additive defects in body curvature and presence of kidney cysts. White arrows show kidney cysts. Statistical comparison of different genotypes was performed using chi-square analysis with p < 0.05 being considered to report significant differences.

(D) Immunoprecipitation (IP) of Flag-tagged Dvl and subsequent Flag-Dvl and HA-Kur wetern blot (WB) reveals physical association between Dvl and Kur.

(E) Immunoprecipitation (IP) of Myc-tagged Dvl domains and subsequent Myc-Dvl and HA-Kur western blot (WB) reveals physical association between Kur and the DEP and PDZ domains but not the DIX domain of Dvl.

(F) Immunoprecipitation (IP) of Flag-tagged Kur and subsequent Flag-Kur and HA-Sea western blot (WB) reveals physical association between Sea and Kur.