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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Clin Cancer Res. 2015 Oct 12;22(5):1197–1210. doi: 10.1158/1078-0432.CCR-14-3379

Figure 4.

Figure 4

Effects of SP1 knockdown with or without overexpression of p53 in MPM cells. (*, P < 0.05; **, P < 0.01; ***, P < 0.001.)

A). Left panel: SP1 depletion and p53 over-expression significantly inhibits in-vitro proliferation (left panel), and decreases volume and mass of MES7 xenografts (middle and right panels, respectively).

B). Venn diagram (left panel), and corresponding heatmap (middle panel) corresponding to genes regulated by MM and overexpression of p53 in SP1-depleted MES1 and MES7 cells. Ingenuity pathway analysis of genes commonly regulated by in MES1 and/or MES7 cells by MM and SP1KD/p53OEX (right panel).

C). qRT-PCR and immunoblot analysis (left and right panels, respectively) demonstrating the effects of SP1 knockdown or overexpression of p53 on genes targeted by MM in cultured MES1 and MES7 cells.