Figure 3.

Antagonism of Y-QA31 for α_1A adrenoceptor and partial agonism for 5-HT_1A receptor. (A) [³⁵S]GTPγS-binding assay was used for the 5-HT_1A receptor. Data are representative of three independent experiments. (B) An intracellular Ca²⁺ release assay was used for the α_1A adrenoceptor. F₀: the basis fluorescence intensity that did not receive drug treatment, F₁: the peak fluorescence intensity after drug treatment. F₁/F₀: the ratio of the fluorescence intensity after drug treatment compared to that before drug treatment. The data are representative of five to six independent experiments. ^cP<0.01 vs control, ^fP<0.01 vs NE; one way ANOVA followed by Bonferroni's multiple comparisons test. NE, norepinephrine, 10 μmol/L; Y-QA31, 10 μmol/L.