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. 2016 Jan 25;37(3):312–321. doi: 10.1038/aps.2015.106

Figure 1.

Figure 1

Desensitization properties of the α7-nAChR in cultured hippocampal neurons. The choline-induced (10 mmol/L for 1 s) response was reversibly blocked by methyllycaconitine (MLA, 10 nmol/L) (A) or irreversibly blocked by α-bungarotoxin (α-Bgt, 100 nmol/L) (B). (C) The currents evoked by paired pulse of choline at different intervals. (D) Curve showing the recovery from desensitization of the α7-nAChR after a choline pulse (n=6 cells). (E) Test choline-induced currents (10 mmol/L for 0.1 s) obtained before, during 0.1 mol/L choline perfusion, and 10 min after agonist washout. (F) Time course plot showing the change in choline-induced currents before, during, and after 0.1 mol/L choline perfusion (n=6 cells). 0 min is the beginning of choline perfusion. (G) Test choline-induced currents obtained before and 10 min after the perfusion of varying concentrations of choline. (H) The plot showing concentration dependence of the desensitization of the α7-nAChR that is induced by choline perfusion. Cho, choline; ICho, choline-evoked current. Holding potential was −80 mV. Data are shown as the mean±SEM.