Figure 3.

MLA abolishes the modulation of the GABA_A or NMDA receptor by the choline pulse in cultured hippocampal neurons. Typical muscimol- (A) or NMDA- (C) evoked currents obtained before or after a choline pulse, as well as after a wash, in normal or 10 nmol/L MLA-containing solution. Corresponding bar graphs show the changes in muscimol- (B, n=5 cells) and NMDA- (D, n=5 cells) induced responses before and after a choline pulse in different bath solutions. Data are shown as the mean±SEM. ^cP<0.01 vs control in corresponding solution, Student's t-test. Cho, choline; Mus, muscimol; I_Mus, muscimol-evoked current; I_NMDA, NMDA-evoked current. Holding potential was −80 mV.