Figure 4.

Chronic choline perfusion selectively modulates the GABA_A or NMDA receptor in cultured hippocampal neurons. Muscimol- (A) and NMDA- (C) evoked currents obtained before and during 0.1 or 0.5 mmol/L choline perfusion, and 10 min after agonist washout. Time course plots indicate the changes in muscimol- (B, n=7 cells) and NMDA- (D, n=5 cells) induced currents before, during, and after choline perfusion. 0 min is the beginning of choline perfusion. Data are shown as the mean±SEM. ^bP<0.05, ^cP<0.01 vs control, one-way ANOVA, LSD post hoc test. Cho, choline; Mus, muscimol; I_Mus, muscimol-evoked current; I_NMDA, NMDA-evoked current. Holding potential was −80 mV.