Figure 4.

DW10075 suppressed the in vitro proliferation of a panel of tumor cells and exerts antitumor activity in U87-MG xenograft tumor in nude mice model through anti-angiogenetic and anti-proliferative effects. (A) DW10075 could inhibit the growth of tumor cells in vitro, but need much higher concentration. The IC₅₀ values of DW10075 are plotted as the mean±SD and all estimated values are from three independent experiments. (B) DW10075 inhibits growth of U87-MG human glioblastoma tumors in nude mice. Tumor volume was measured on the indicated days with the mean tumor volume indicated for groups. (C) DW10075 had no distinct impact on the weight of treated group animals. Mean weight±SD were shown and the initial weight is 22±2 g, following the treatment of DW10075 for 14 d, the weight is still kept in this range. (D) The expression of CD31 and Ki67 were significant reduced in the 500 mg/kg po qd DW10075 group in the U87-MG xenograft. Evaluation of CD31 and Ki-67 expression in tumor tissue of treated or vehicle control groups by IHC on d 14. (E) Quantitative analysis of the CD31 and Ki67 expression in the 500 mg/kg po qd DW10075 group in the U87-MG xenograft. The quantity of the expression was evaluated by IOD sum using Image-Pro Express. Mean±SD. n=3. ^bP<0.05, vs vehicle control.