Figure 1.
OPD increases CYP2J3 expression and 14,15-DHET levels in cardiomyocytes. H9c2 cells were treated as described in the Methods. CYP2J3 expression was quantified by qRT-PCR (A) and Western blotting (B). (C) 14,15-DHET levels were measured with an ELISA. (D) H9c2 cells were pretreated with OPD as indicated in the Methods, and 14,15-DHET levels were increased compared with the PPOH-treated group. Data are presented as the mean±SEM. n=3–4. bP<0.05, cP<0.01 vs the controls; eP<0.05 vs the PPOH-treated group.