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. 2016 Feb 17;6(4):558–570. doi: 10.7150/thno.14693

Figure 3.

Figure 3

Overexpression of Hsp27 promotes HCC cell migration and invasion. (A-B) The detection of overexpression of Hsp27 in SK-Hep1 and SMMC7721 cells by real-time PCR and western blotting. (C) A representative result of the CCK-8 assays for the effects of Hsp27 overexpression on proliferation of SK-Hep1 and SMMC7721 cells. (D) Transwell assays for the effects of Hsp27 overexpression on migratory and invasive potentials of MHCC97H and HCCLM3 cells (magnification, ×100). (E-F) Results of in vivo fluorescence imaging and the incidence of lung metastasis in the indicated groups. (G-H) Representative images for lung and liver metastatic nodules in indicated groups (magnification, ×100, ×200). (I) The number of lung metastatic nodules was quantified on serial sections of H&E staining. *P < 0.05, ***P < 0.001.